Plasmid
Part:BBa_K3041014:Design
Designed by: Jo-Anne Verschoor Group: iGEM19_Leiden (2019-10-07)
ipET28
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 168
Illegal BamHI site found at 58 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 200
Illegal NgoMIV site found at 1788
Illegal NgoMIV site found at 1948
Illegal NgoMIV site found at 4995 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 2868
Design Notes
A new multiple cloning site was created using the biobrick setup when designing it had to be taken into account that the RBS would be added to the multiple-cloning site otherwise it would have been lost during cloning.
Source
Sequence derived from pET28a(+) of add gene.