Plasmid

Part:BBa_K3041014:Design

Designed by: Jo-Anne Verschoor   Group: iGEM19_Leiden   (2019-10-07)


ipET28


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 168
    Illegal BamHI site found at 58
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 200
    Illegal NgoMIV site found at 1788
    Illegal NgoMIV site found at 1948
    Illegal NgoMIV site found at 4995
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2868


Design Notes

A new multiple cloning site was created using the biobrick setup when designing it had to be taken into account that the RBS would be added to the multiple-cloning site otherwise it would have been lost during cloning.


Source

Sequence derived from pET28a(+) of add gene.

References